Solutions

6X gel loading dye (makes 100ml)
Dissolve in 49ml diH20:
0.25g bromophenol blue
0.25 xylene cyanol
1 ml Tris
Stir in 50ml glycerol.

0.5X TBE (buffer for running gels)
Dissolve in 800ml distilled H20: (1) 108g Tris base and (2) 55g Borate (Boric Acid)
Add 40ml 0.5M EDTA (pH 8.0)
Add diH20 to bring to 1L (=10X TBE)
Add 19L distilled H20 to solution (=0.5X TBE)

1M Tris (pH 7.4)
Dissolve 121.1g Tris Base in 800ml diH20.
Adjust pH with concentrated HCl.
Bring volume to 1L with diH20 and check pH.

0.5M EDTA (pH 8.0)
Dissolve 186.1g EDTA in 800ml diH20.
Stir vigorously on warmed stirplate to dissolve (this will take some time)
Adjust pH with concentrated NaOH.
Bring volume to 1L with diH20 and check pH.

1X TE (pH 7.4)
10ml 1M Tris (pH 7.4)
2ml 0.5M EDTA (pH 8.0)
Bring volume to 1L with diH20 and check pH.

Posted by Julienne Ng – Dec. 4th, 2009

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